Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

基于声学调制解调器的声速剖面遥测技术     

余平  廖开训  陶军  肖波 《海洋技术学报》2006,25(3):90-92

声速是海洋声呐测量中最重要的声学参数之一。拖缆式声速剖面仪可提供实时测量功能,但其缆长不能满足深海测量要求;自容式声速剖面仪可满足深海测量要求,但工作效率低。如何提高野外深海声速剖面测量工作效率是深海调查一项非常有意义的工作。文中详细介绍了基于声学调制解调器的声速剖面遥测系统的主要结构和功能,揭示了水声通信技术在深海非铠装缆测量中的应用优势。  相似文献   

三次样条函数在海洋水文资料整理中的应用     

郑东 《中国海洋大学学报(自然科学版)》1989,(Z1)

本文引入三次样条函数插值法,并尝试用于海洋资料处理。根据渤海及北黄海部分海洋站的历史资料进行了试验。用SHARP—PC—1211袖珍计算机进行计算,并将资料处理的结果与传统的过程曲线法订正值作了比较。结果表明,二者之间的误差不大,而样条函数拟合更好。作者认为三次样条函数插值法是可行的,在处理海洋资料时间序列中是有效的。在有温跃层的地方,如何应用,本文也给出了必要的说明。  相似文献   

TSDV: A GIS tool for inspecting trawl survey data     

Fortunati  L.; Garofalo  G.; Demontis  R. 《ICES Journal of Marine Science》2002,59(1):168-178

  相似文献   

A Method of Coding and Decoding in Underwater Image Transmission     

程恩 《海洋通报(英文版)》2001,3(2)

A new method of coding and decoding in the system of underwater image transmission is introduced, including the rapid digital frequency synthesizer in multiple frequency shift keying,image data generator, image grayscale decoder with intelligent fuzzy algorithm, image restoration and display on microcomputer.  相似文献   

Systematic marine conservation planning in data-poor regions: Socioeconomic data is essential   总被引：1，自引：0，他引：1  

Natalie C. Ban  Gretchen J.A. Hansen  Michael Jones  Amanda C.J. Vincent   《Marine Policy》2009,33(5):794-800

Systematic planning for conservation is highly regarded but relies on spatially explicit data that are lacking in many areas of conservation concern. The decision support tool Marxan is applied to a reef system in the central Philippines where 30 marine protected areas (MPAs) have been established in communities without much use of biophysical data. The intent was to explore how Marxan might assist with the legally required expansion to protect 15% of marine waters, and how existing MPAs might affect that process. Results show that biophysical information alone did not provide much guidance in identifying patterns of conservation importance in areas where the data are poor. Socioeconomic data were needed to distinguish among possible areas for protection; but here, as elsewhere in marine environments, the availability of such data was very limited. In the final analysis, local knowledge and integrated understanding of socioeconomic realities may offer the best spatially explicit information. The 30 existing MPAs, which encompassed a small proportion of the reef system, did not limit future options in developing a suite of MPAs on a broader scale. Rather, they appeared to generate the support for MPAs that is obligatory for any larger zoning effort. In summary, establishing MPAs based on community-driven criteria has biological and social value, but efforts should be made to collect ecological and socioeconomic data to guide the continued creation of MPAs.  相似文献   

On the geologic structure of the Explora Escarpment (Weddell Sea,Antarctica) revealed by satellite and Shipboard data evaluation     

Ute Christina Herzfeld  Arnold L. Brodscholl 《Marine Geophysical Researches》1994,16(5):325-345

Bathymetric, gravity, and magnetic data from Antarctic expeditions with RV POLARSTERN and satellite altimeter data from the Geosat Geodetic Mission are analysed using methods from geostatistics and geophysical inverse theory.The Explora Escarpment represents the edge between the Antarctic Continental Shelf and the Weddell Abyssal Plain. It is an important link in the reconstruction of Gondwana breakup, but a feature as large as the 2000 m deep Wegener Canyon was only discovered in 1984, when extensive bathymetric, gravimetric, and magnetic surveys with RV POLARSTERN began.Geostatistics, the theory of regionalized variables, is applied to integrate dense surveys of Wegener Canyon and sparse observations in adjacent areas into maps with full coverage of the 230 km by 330 km area at 10°–20° W/70°–72° S. The resultant highresolution bathymetric and gravity maps reveal detailed structures of the Explora Escarpment. Using geophysical inversion, the gravity terrain effect is calculated. Satellite data are used for their better coverage, but have much lower resolution. Nevertheless, the structures of Wegener Canyon and other more prominent features appear with surprisingly good correlation also in the Geosat altimeter data. While it was initially supposed that Wegener Canyon is purely an erosional structure, the magnetic map now provides evidence of the canyon's tectonic origin.  相似文献   

French marine technology policy : Industrializing ocean depths     

Daniel Spagni  Luke Georghiou  Michael Gibbons 《Marine Policy》1985,9(4):280-291

This paper outlines the structure of the institutions involved in scientific and technological development in France. It points to new trends in French marine policy, and gives details of the country's offshore petroleum technology and nodule mining.  相似文献   

我国海水制盐工艺改革的设计研究     

周仲怀  徐丽君  于银亭  王建华  于廷芳  王继业  殷丽 《海洋科学》1997,21(6):43-46

从技术上和工艺上的可能性、可行性和合理性，论证了海水制盐工艺改革的必要性，并且提出了实现海水制盐工艺改革的各种设计方案。它对我国的海水制盐工业的发展将具有深远意义。  相似文献   

Theoretical aspects of cap-rock and fault seals for single- and two-phase hydrocarbon columns   总被引：6，自引：0，他引：6  

N.L. Watts   《Marine and Petroleum Geology》1987,4(4)

Cap-rock seals can be divided genetically into those that fail by capillary leakage (membrane seals) and those whose capillary entry pressures are so high that seal failure preferentially occurs by fracturing and/or wedging open of faults (hydraulic seals). A given membrane seal can trap a larger oil column than gas column at shallow depths, but below a critical depth (interval), gas is more easily sealed than oil. This critical depth increases with lower API gravity, lower oil GOR and overpressured conditions (for the gas phase). These observations arise from a series of modelling studies of membrane sealing and can be conveniently represented using pressure/ depth (P/D) profiles through sealed hydrocarbon columns. P/D diagrams have been applied to the more complex situation of the membrane sealing of a gas cap underlain by an oil rim; at seal capacity, such a two-phase column will be always greater than if only oil or gas occurs below the seal.These conclusions contrast with those for hydraulic seals where the seal capacity to oil always exceeds that for gas. Moreover, a trapped two-phase column, at hydraulic seal capacity will be less than the maximum-allowed oil-only column, but more than the maximum gas-only column. Unlike membrane seals, hydraulic seal capacity should be directly related to cap-rock thickness, in addition to the magnitude of the minimum effective stress in the sealing layer and the degree of overpressure development in the sequence as a whole.Fault-related seals are effectively analogous to membrane cap-rocks which have been tilted to the angle of the fault plane. Consequently, all of the above conclusions derived for membrane cap-rocks apply to both sealing faults sensu stricto (fault plane itself seals) and juxtaposition faults (hydrocarbon trapped laterally against a juxtaposed sealing unit). The maximum-allowed two-phase column trapped by a sealing fault is greater than for equivalent oil-only and gas-only columns, but less than that predicted for a horizontal membrane cap-rock under similar conditions. Where a two-phase column is present on both sides of a sealing fault (which is at two-phase seal capacity), a deeper oil/water contact (OWC) in one fault block is associated with a deeper gas/oil contact (GOC) compared with the adjacent fault block. If the fault seal is discontinuous in the gas leg, however, the deeper OWC is accompanied by a shallower GOC, whereas a break in the fault seal in the oil leg results in a common OWC in both fault blocks, even though separate GOC's exist. Schematic P/D profiles are provided for each of the above situations from which a series of fundamental equations governing single- and two-phase cap-rock and fault seal capacities can be derived. These relationships may have significant implications for exploration prospect appraisal exercises where more meaningful estimates of differential seal capacities can be made.The membrane sealing theory developed herein assumes that all reservoirs and seals are water-wet and no hydrodynamic flow exists. The conclusions on membrane seal capacity place constraints on the migration efficiency of gas along low-permeabiligy paths at depth where fracturing, wedging open of faults and/or diffusion process may be more important. Contrary to previous assertions, it is speculated that leakage of hydrocarbons through membrane seals occurs in distinct pulses such that the seal is at or near the theoretically calculated seal capacity, once this has been initially attained.Finally, the developed seal theory and P/D profile concepts are applied to a series of development geological problems including the effects of differential depletion, and degree of aquifer support, on sealing fault leakage, and the evaluation of barriers to vertical cross-flow using RFT profiles through depleted reservoirs. It is shown that imbibition processes and dynamic effects related to active cross-flow across such barriers often preclude quantitative analysis and solution of these problems for which simulation studies are usually required.  相似文献